Composite

Part:BBa_K4642017

Designed by: Ryan Yapa   Group: iGEM23_City-of-London-UK   (2023-09-28)


T7 / Gen4 486-19b-1260a Double AND-Gate / GFP / Double Terminator

This composite part is used for testing the presence of hsa-miR-486, hsa-miR-19b and hsa-miR-1260a. It has a T7 promoter for expression of functional RNA BBa_K1614000. This is followed by the code for our toehold switch BBa_K4642014. Placing a reporter protein downstream of this toehold switch part will mean that when our trigger RNAs are present in high enough quantities, the protein in the CDS will be translated and the reporter protein can be observed. Our chosen reporter protein is GFP, BBa_I746916. The fluorescence produced can be detected with a luminometer to affirm a positive test.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 178


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Parameters
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